鸭源鸡杆菌ompW基因缺失菌株的构建及其药物敏感性分析

doi:10.11843/j.issn.0366-6964.2017.07.015

Abstract

Abstract:

The study was conducted to explore the role of ompW gene in the drug resistance of Gallibacterium anatis (G. anatis). The ompW gene knock-out mutant of G. anatis was constructed by natural transformation. The competent cells of G. anatis were induced following transfer of cells to M-IV medium from colonies on a blood agar plate. Then, transformation was performed by incubating the M-IV-compentent cells together with linear DNA fragment consisting of homologous arms and cmp^r gene. The transformants were identified by using PCR, SDS-PAGE and Western blot analysis. The MIC-determinations of 12 kinds of antibacterial agents were performed for both wild type and mutant ΔompW G. anatis using Mueller Hinton-Ⅱ. The ompW gene deletion strain ΔompW was successfully constructed. Comparing with wild type G. anatis, the MICs of terramycin and tetracycline for ΔompW were decreased to 1/8-fold (16/128) and 1/32-fold (4/128), respectively. The MICs of other drugs in this study showed no obvious change. In conclusion, ompW deletion strain of G. anatis was constructed by natural transformation for the first time in this study. OmpW plays an important role in the formation of tetracyclines resistance phenotype of G. anatis strains.

CLC Number:

S855.12

PENG Zhi-feng, CUI Dan-dan, YANG Xia, JING Wen-xian, WANG Zhong-tian, WANG Kun-peng, WANG Chuan-qing. Construction of ompW Gene Deletion Strain of Gallibacterium anatis and Its Susceotibility to Antibacterial Agents[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1306-1313.

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Construction of ompW Gene Deletion Strain of Gallibacterium anatis and Its Susceotibility to Antibacterial Agents

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